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Lactose hydrolysis by β-galactosidase enzyme: optimization using response surface methodology.
- Source :
- Ecotoxicology & Environmental Safety; Nov2015, Vol. 121, p244-252, 9p
- Publication Year :
- 2015
-
Abstract
- In the present study, it was aimed to optimize the process of lactose hydrolysis using free and immobilized β-galactosidase to produce glucose and galactose. Response surface methodology (RSM) by central composite design (CCD) was employed to optimize the degree of hydrolysis by varying three parameters, temperature (15–45 °C), solution pH (5–9) and β-galactosidase enzyme concentration (2–8 mg/mL) for free mode of analysis and sodium alginate concentration (2–4%), calcium chloride concentration (3–6%) and enzyme concentration (2–8 mg/mL) for immobilized process. Based on plots and variance analysis, the optimum operational conditions for maximizing lactose hydrolysis were found to be temperature (35.5 °C), pH (6.7) and enzyme concentration (6.7 mg/mL) in free mode and sodium alginate concentration (3%), calcium chloride concentration (5.9%) and enzyme concentration (5.2 mg/mL) in immobilized mode. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01476513
- Volume :
- 121
- Database :
- Supplemental Index
- Journal :
- Ecotoxicology & Environmental Safety
- Publication Type :
- Academic Journal
- Accession number :
- 109320673
- Full Text :
- https://doi.org/10.1016/j.ecoenv.2015.03.024