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First characterization of the CD4 and CD8 T-cell responses to QuantiFERON-TB Plus.
- Source :
- Journal of Infection; Dec2016, Vol. 73 Issue 6, p588-597, 10p
- Publication Year :
- 2016
-
Abstract
- <bold>Introduction: </bold>QuantiFERON®-TB Gold Plus (QFT-Plus) is the new generation of QuantiFERON-TB Gold In-Tube test to identify latent tuberculosis infection (LTBI). QFT-Plus includes TB1 and TB2 tubes which contain selected Mycobacterium tuberculosis (Mtb) peptides designed to stimulate both CD4 and CD8 T-cells. Aim of this study is the flow cytometric characterization of the specific CD4 and CD8 T-cell responses to Mtb antigens contained within QFT-Plus.<bold>Methods: </bold>We enrolled 27 active tuberculosis (TB) patients and 30 LTBI individuals. Following stimulation with TB1 and TB2, antigen-specific T-cells were characterized by flow cytometry. Data were also correlated with the grade of TB severity.<bold>Results: </bold>TB1 mainly elicited a CD4 T-cell response while TB2 induced both CD4 and CD8 responses. Moreover, the TB2-specific CD4 response was detected for both active TB and LTBI patients, whereas the TB2-specific CD8 response was primarily associated with active TB (p = 0.01).<bold>Conclusions: </bold>To our knowledge, we report the first characterization of the CD4 and CD8 T-cell response to QFT-Plus. CD8 T-cell response is mainly due to TB2 stimulation which is largely associated to active TB. These results provide a better knowledge on the use of this assay. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01634453
- Volume :
- 73
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Journal of Infection
- Publication Type :
- Academic Journal
- Accession number :
- 119777966
- Full Text :
- https://doi.org/10.1016/j.jinf.2016.09.008