Anti-Helicobacter pylori effect of various extracts of ixeris chinensis on inflammatory markers in human gastric epithelial AGS cells.

Ixeris chinensis (Thunb.) Nakai (Taiwan) is a traditional herbal medicine used for treating various ailments, such as stomach ache, common cold and diarrhoea. The objective of the present study was to evaluate the antioxidative, anti-inflammatory and anti-adhesive effect of I. chinensis on Helicobacter pylori ( H. pylori )-induced gastric adenocarcinoma in an AGS cell model. Three types of I. chinensis extract were used: boiling water extract (BWE), ethanol extract (EE) and chloroform extract (CE) to check anti- H. pylori activity on the AGS cell line. Phytochemical and HPLC analysis were performed. In vitro antioxidant activity, including TEAC, DPPH and H 2 O 2 scavenging abilities, was evaluated. In addition, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined, and an MTT assay determined survival rate. Furthermore, interleukin-8 (IL-8), tumor necrosis factor (TNF-α) and nitric oxide (NO) levels were measured. The BWE showed better antioxidative activity than the EE and CE. The lag time of human low-density lipoprotein (LDL) oxidation was significantly increased by BWE, EE and CE. However, CE showed maximum antibacterial (MIC, MBC), anti-adhesive (urease test) and anti-inflammatory (IL-8, NO, TNF-α) activities. Based on the above findings, I. chinensis extract (BWE and CE) clearly shows antioxidative, anti-inflammatory and anti-adhesive effects on H. pylori -induced gastric adenocarcinoma in the AGS cell model. Hence, I. chinensis might be used to combat against H. pylori- induced gastric cancer with standard chemotherapeutic agents. [ABSTRACT FROM AUTHOR]