Back to Search
Start Over
Direct interaction between VRN1 protein and the promoter region of the wheat FT gene.
- Source :
- Genes & Genetic Systems; Feb2018, Vol. 93 Issue 1, p25-29, 5p
- Publication Year :
- 2018
-
Abstract
- The wheat florigen gene Wheat FLOWERING LOCUS T (WFT, which is identical to VRN3) is an integrator of the vernalization, photoperiod and autonomous pathways in wheat flowering. Many studies have indicated that VERNALIZATION 1 (VRN1) directly or indirectly up-regulates WFT expression in leaves. VRN1 encodes an APETALA1/FRUITFULL-like MADS box transcription factor that is up-regulated by vernalization and aging, leading to promotion of flowering. In this study, the VRN1 protein was expressed as a His-Tag fusion protein in Escherichia coli and used in an electrophoretic mobility shift assay (EMSA). The results from the EMSA indicated that the VRN1 protein directly binds to the CArG-box in the promoter region of WFT, suggesting the direct up-regulation of WFT by VRN1 in the leaves of wheat plants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13417568
- Volume :
- 93
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Genes & Genetic Systems
- Publication Type :
- Academic Journal
- Accession number :
- 130768051
- Full Text :
- https://doi.org/10.1266/ggs.17-00041