Fungal mediated biotransformation reduces toxicity of arsenic to soil dwelling microorganism and plant.

Rhizospheric and plant root associated microbes generally play a protective role against arsenic toxicity in rhizosphere. Rhizospheric microbial interaction influences arsenic (As) detoxification/mobilization into crop plants and its level of toxicity and burden. In the present investigation, we have reported a rhizospheric fungi Aspergillus flavus from an As contaminated rice field, which has capability to grow at high As concentration and convert soluble As into As particles. These As particles showed a reduced toxicity to soil dwelling bacteria, fungi, plant and slime mold. It does not disrupt membrane potential, inner/outer membrane integrity and survival of the free N 2 fixating bacteria. In arbuscular mycorrhiza like endophytic fungi Piriformospora indica , these As particles does not influence mycelial growth and plant beneficial parameters such as phosphate solubilizing enzyme rAPase secretion and plant root colonization. Similarly, it does not affect plant growth and chlorophyll content negatively in rice plant. However, these As particles showed a poor absorption and mobilization in plant. These As particle also does not affect attachment process and survival of amoeboid cells in slime mold, Dictyostelium discoideum. This study suggests that the process of conversion of physical and chemical properties of arsenic during transformation, decides the toxicity of arsenic particles in the rhizospheric environment. This phenomenon is of environmental significance, not only in reducing arsenic toxicity but also in the survival of healthy living organism in arsenic-contaminated rhizospheric environment. Image 1 • A rhizospheric fungus from rice has ability to bio-transform soluble arsenic into immobilized arsenic particle. • These arsenic particles are non-toxic for bacteria and fungi and does not influence their growth negatively. • Arsenic particles have reduced mobilization to plant and does not negatively impact beneficial plant-microbe interaction. • These particles does not influence adversely on the growth and attachment process of soil residing amoeba (slime mold). • Bio-transformed arsenic particles are more compatible to rhizospheric environment than soluble arsenic. [ABSTRACT FROM AUTHOR]