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Effect of Apatinib on Invasion and Migration of Lung Cancer Cells and Its Mechanism.
- Source :
- Chinese Journal of Lung Cancer; 5/20/2019, Vol. 22 Issue 5, p264-270, 7p, 1 Diagram, 3 Graphs
- Publication Year :
- 2019
-
Abstract
- Background and objective Lung cancer is one of the most deadly cancers in the world for human. In recent years, the effect of targeted therapy has become increasingly significant. Apatinib is a multi-target anti-tumor drug that is currently under study. The purpose of this study is to investigate the effects of Apatinib on the biological characteristics of lung cancer cells and its possible mechanism. Methods Lung cancer cell lines H1299 and H3255 were cultured in vitro. The effects of Apatinib on proliferation, migration and invasion of H1299 and H3255 cells were detected by cell proliferation assays wound healing assays and Transwell assays. The protein expression related to cancer angiogenesis and invasion was detected by Western blot. Results Apatinib significantly inhibited the proliferation, migration and invasion of H1299 and H3255 in a concentration-dependent manner. Western blot showed that with the increasing of drug concentration, VEGF, VEGFR2, N-cadherin, MMP9, MMP2 and Vimentin were down-regulated, and E-cadherin were up-regulated. Conclusion Apatinib can inhibit the invasion and migration of lung adenocarcinoma cells H1299 and H3255. By regulation of epithelial-mesenchymal transition and the expression of matrix metalloproteinase-related proteins. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL proliferation
CELL adhesion molecules
CELL lines
CELL migration
CELL motility
CLINICAL trials
CYTOSKELETAL proteins
GROWTH factors
LUNG tumors
MICROBIOLOGICAL assay
WESTERN immunoblotting
PROTEIN-tyrosine kinase inhibitors
VASCULAR endothelial growth factors
IN vitro studies
PHARMACODYNAMICS
THERAPEUTICS
Subjects
Details
- Language :
- Chinese
- ISSN :
- 10093419
- Volume :
- 22
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- Chinese Journal of Lung Cancer
- Publication Type :
- Academic Journal
- Accession number :
- 136520588
- Full Text :
- https://doi.org/10.3779/j.issn.1009-3419.2019.05.02