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Gene cloning of the pink-colored protein from Pleurotus salmoneostramineus (PsPCP) and its species-specific chromoprotein are effective for colorization of the fruit body.
- Source :
- Bioscience, Biotechnology & Biochemistry; Jul2019, Vol. 83 Issue 7, p1354-1361, 8p
- Publication Year :
- 2019
-
Abstract
- Pleurotus salmoneostramineus is a pink mushroom. This pink color is a protein and forms a complex with 3H-indol-3-one. The gene encoding the pink-colored protein from P. salmoneostramineus (PsPCP) was cloned, and its sequence was elucidated as a 681-bp. The ORF encodes 226 amino acid residues. The amino acid sequence of the protein did not show any significant homology in the DDBJ/EMBL/GenBank databases. Recombinant PsPCP was expressed as the soluble form in E. coli. The reaction mixture of purified recombinant PsPCP and 3H-indol-3-one showed a pink color as the native pigment. A real-time PCR analysis revealed the strong expression of PsPCP in the primordium formation stage of the life cycle of the fungus; however, its expression decreased with the maturation of the fruit body. A comparison of PsPCP gene expression profiles between two strains revealed high levels in the dark-colored strain. Gene cloning of the pink-colored protein from Pleurotus salmoneostramineus (PsPCP) and its species-specific chromoprotein are effective for colorization of the fruit body. [ABSTRACT FROM AUTHOR]
- Subjects :
- CLONING
CHROMOPROTEINS
POLYMERASE chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 09168451
- Volume :
- 83
- Issue :
- 7
- Database :
- Supplemental Index
- Journal :
- Bioscience, Biotechnology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 136909569
- Full Text :
- https://doi.org/10.1080/09168451.2019.1611406