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Anti-inflammatory action of YHQ by regulating 5-LOX/COX-2/NF-κB/MAPKs/Akt signaling pathways in RAW 264.7 macrophage cells.
- Source :
- Journal of Herbal Medicine; Sep2019, Vol. 17, pN.PAG-N.PAG, 1p
- Publication Year :
- 2019
-
Abstract
- Yan-Hou-Qing (YHQ), a traditional Chinese medicine (TCM) formula including fifteen herbal medicines has been used for acute pharyngitis and cough treatment in Oriental medicine. However, anti-inflammatory activities of YHQ are poorly understood. The anti-inflammatory activities of YHQ were evaluated via enzyme-linked immunosorbent assay (ELISA), and the underlying mechanisms were further determined using western blot in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells in vitro. Anti-inflammation study revealed that YHQ inhibits the release of prostaglandin E 2 (PGE 2) potently by suppressing the protein expression of cyclooxygenase-2 (COX-2) and leukotriene B 4 (LTB 4) by reducing arachidonate 5-lipoxygenase (5-LOX) in LPS-stimulated RAW 264.7 macrophages. Further study demonstrated that YHQ inhibits the production of the pro-inflammatory cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 macrophages by attenuating the phosphorylation p65 subunit of nuclear factor-kappaB (NF-κB). The suppressive effects of YHQ on LPS-stimulated inflammatory cytokines and mediators can be attributed to the suppression of YHQ on the phosphorylation of Akt and c-Jun N-terminal kinases (JNK), extracellular signal-regulated kinase (ERK), and p38 MAPKs. This study suggested that YHQ can be a preventive and potent therapeutic candidate for the management of inflammatory-mediated immune disorders. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22108033
- Volume :
- 17
- Database :
- Supplemental Index
- Journal :
- Journal of Herbal Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 140375415
- Full Text :
- https://doi.org/10.1016/j.hermed.2019.100269