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Supercritical CO2 extraction of Aurantiochytrium sp. biomass for the enhanced recovery of omega-3 fatty acids and phenolic compounds.
- Source :
- Journal of CO2 Utilization; May2020, Vol. 38, p24-31, 8p
- Publication Year :
- 2020
-
Abstract
- • SFE of microalgae Aurantiochytrium sp. and Soxhlet extraction (n-hexane & dichloromethane). • Maximization of total extraction yield, DHA & total phenolics content, & antioxidant capacity. • Design of experiments with 3 factors and 2 levels (200−300 bar, 40−80 °C, 6–12 g C O 2 m i n - 1). • Obtained DHA content 3.5-fold richer than fish oil, and 7.9-fold richer than other microalgaes. The microalgae Aurantiochytrium sp. is a strong alternative source of ω-3 fatty acids, including docosahexaenoic acid (DHA). This work encompasses the optimization of SFE conditions to maximize the total extraction yield η T o t a l , DHA content (C D H A), total phenolics content (T P C), and antioxidant capacity (A O C) of the extracts produced from Aurantiochytrium sp. biomass. A full factorial experimental plan was performed, comprising three factors (pressure, temperature, and flow rate) and two levels (200−300 bar, 40−80 °C, and 6–12 g m i n - 1 , respectively). The maximum and minimum experimental results were η T o t a l = 2.1 and 13.4 wt.%, C D H A = 27.3 and 39.3 wt.%, T P C = 1.19 and 2.24 m g G A E g e x t r a c t - 1 , and A O C = 0.3 and 1.4 m g T E A C g e x t r a c t - 1. Under the studied experimental conditions, increasing pressure up to 300 bar is the optimum to rise both η T o t a l and C D H A. Temperature increase from 40 to 80 °C leads to opposing effects: it favors the concentration of phenolics in the supercritical extracts at the expenses of decreasing DHA content and total yield. Surface models were adjusted to η T o t a l , C D H A and T P C data, and the goodness of the fits ranged from coefficients of determination of 0.752-0.711 (T P C) to 0.997-0.994 (C D H A). Under optimized conditions, supercritical extracts exhibited a DHA content more than 3.5-fold richer than fish oil, and 7.9-fold richer than the best alternative microalgae species (Pavlova lutheri) found in the literature. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22129820
- Volume :
- 38
- Database :
- Supplemental Index
- Journal :
- Journal of CO2 Utilization
- Publication Type :
- Academic Journal
- Accession number :
- 143060246
- Full Text :
- https://doi.org/10.1016/j.jcou.2020.01.014