Characterization of the interaction between hen egg white lysozyme and ovalbumin: Interaction between lysozyme and ovalbumin.

As two major proteins of egg white, the interaction between lysozyme (LYZ) and ovalbumin (OVA) has been studied. In the present research, the mechanism and kinetics of the interaction between LYZ and OVA were studied using several methods, including cyclic voltammetry (CV), dual polarization interferometry (DPI) and surface plasmon resonance (SPR), and the effect of their interaction on the activity of LYZ was investigated using a bacteriolytic activity assay. Cyclic voltammograms showed that there was an interaction between these two proteins. DPI results indicated that the interaction is a type of chimeric mode, and the K A and K D of the interaction were 498 and 2.01 × 10⁻³ mol/L, respectively, calculated from the fitting of the surface plasmon resonance curve. The bacteriolytic activity assay showed that the interaction between these two proteins reduced the bacteriolytic activity of LYZ. [ABSTRACT FROM AUTHOR]