Assessment spermatogenic cell apoptosis and the transcript levels of metallothionein and p53 in Meretrix meretrix induced by cadmium.

Cadmium (Cd) has been widely used in industry and can accumulate in the water, soil, and food. Meretrix meretrix is one of the marine shellfishes cultivated for economic purpose in China. The increasing Cd levels in coastal marine water could adversely affect the economic benefits of shellfish cultivation. In the present study, M. meretrix were exposed to different Cd²⁺ concentrations (0, 1.5, 3, 6, and 12 mg L^-1) for 5 d to evaluate the effects of Cd on spermatogenic cell. The Cd accumulation, survival rate and the indices of oxidative stress and apoptosis were determined in the spermatogenic cells of M. meretrix. The expression levels of p53 and metallothionein (MT) mRNA were also measured in the spermatogenic cells. Cd accumulation and the mortality rate of spermatogenic cells were found to increase in a dose-response manner with Cd²⁺ concentrations. Histopathology changes, especially the damage of membranous structure, were more severe as the Cd²⁺ levels in the testis became higher. The indexes of oxidative stress, including reactive oxygen species, malondialdehyde, protein carbonyl derivates and DNA-protein crosslinks all increased after exposure to Cd²⁺. However, the total antioxidant capacity gradually decreased with the increasing Cd²⁺ concentration. In addition, exposure to Cd²⁺ increased the apoptotic rate and caspase-3 and 9 activities but decreased the level of mitochondrial membrane potential and cytochrome C oxidase in the spermatogenic cells. MT mRNA expression increased in lower Cd²⁺ concentration treated groups whereas decreased in higher groups, while the p53 mRNA expression increased in a dose-response manner with Cd²⁺ and was positively correlated with the oxidative damage indices. These results indicated that Cd²⁺ caused oxidative stress and p53 induced apoptosis in the spermatogenic cells, and thus decreased the survival rate of sperm cells. This finding highlights that Cd can reduce the reproductive capacity of M. meretrix , thus threatening to wild shellfish populations and reducing the efficiency of shellfish farming. • Exposure to Cd increased mortality rate of spermatogenic cells in Meretrix meretrix. • Exposure to Cd induced oxidative stress and apoptosis in the spermatogenic cells. • Exposure to 3 mg L⁻¹ Cd increased the MT mRNA level while 12 mg L⁻¹ Cd decreased it. • The expression level of p53 mRNA was correlated with the oxidative stress indices. [ABSTRACT FROM AUTHOR]