Uptake, translocation and metabolism of imidacloprid loaded within fluorescent mesoporous silica nanoparticles in tomato (Solanum lycopersicum).

Fluorescence-labeling technology has been widely used for rapid detection of pesticides in agricultural production. However, there are few studies on the use of this technology to investigate pesticide uptake and transport in plants with fluorescent nanoparticle formulations. Here, we investigated uptake, transport, accumulation and metabolism of imidacloprid loaded in fluorescent mesoporous SiO 2 nanoparticles (Im@FL-MSNs) in tomato plants, and compared the results with the pesticide application in granular formulation. The results revealed that Im@FL-MSNs applied via root uptake and foliar spray both could effectively transport in tomato plants and carry the imidacloprid to plant tissues. Neither Im@FL-MSNs nor imidacloprid was detected inside of tomato fruits from root uptake or foliar spray applications. Compared with the foliar application of granular formulation, imidacloprid in Im@FL-MSNs demonstrated a higher concentration in leaves (1.14 ± 0.07 mg/kg > 1.08 ± 0.04 mg/kg, 1.13 ± 0.09 mg/kg > 1.11 ± 0.02 mg/kg), longer half-life (0.84 d < 1.31 d, 0.90 d < 1.36 d) and small numbers of metabolites formed. These results suggest that mesoporous silica nanoparticles could serve as an effective and efficient pesticide carrier for achieving the high use efficiency in plant protection. The information is also helpful to guide the pesticide applications and assess the risks associated with environmental quality and dietary consumption of vegetables. [Display omitted] • Dynamic transport of Im@FL-MSNs in tomato was verified by fluorescence technology. • Morphological changes of silica nanoparticles in tomato were studied using TEM. • Metabolic mechanism of imidacloprid was verified by DFT and UPLC-MS/MS. • Im@FL-MSNs has a low risk of application on tomatoes. [ABSTRACT FROM AUTHOR]