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Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model.
- Source :
- Asian Pacific Journal of Tropical Biomedicine; Aug2022, Vol. 12 Issue 8, Preceding p343-350, 6p
- Publication Year :
- 2022
-
Abstract
- Objective: To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×10<superscript>4</superscript> /mL, 5×10<superscript>5</superscript> /mL, 5×10<superscript>6</superscript> /mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22211691
- Volume :
- 12
- Issue :
- 8
- Database :
- Supplemental Index
- Journal :
- Asian Pacific Journal of Tropical Biomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 158295717
- Full Text :
- https://doi.org/10.4103/2221-1691.350182