Impact of silver nanoparticles on multiplication, rooting of shoots and biochemical analyses of date palm Hayani cv. by invitro.

Nano silver has positive effects on the growth and development of in vitro plants. The current study was carried out to investigate the effect of adding different concentrations of nano silver nitrate (0.0, 1.0, 2.0, 3.0 and 4.0 ml/l) to the culture medium in multiplication and rooting stage of the date palm cv. Hayani. The results showed that there was a significant difference of Ag NPs concentrations in regeneration stage as it increased the number and length of shoots at 3.0 ml/l concentrations compared to the control treatment. The outcome of shoots multiplication experiment indicated that Ag NPs concentration had a significant positive effect on studied traits, when the 3.0 ml/l of Ag NPs added to MS basal medium supplemented with BA at 2.0, GA 3 at 0.5 and NAA at 0.1 (mg/l) gave the highest shoots and leaves number as it reached 10.74 shoots and 30.11 leaf/culture with length 6.60 cm, respectively in comparison with lowest results recorded 2.50 shoot/culture, 10.70 leaf/culture with length 2.00 cm, respectively with the control treatment. All shoots were cultivated in a new medium supplemented with NAA at 1.0 mg/l and various concentrations of Ag NPs to study root formation. The findings indicated that the optimal medium for root formation was one that included Ag NPs at 3.0 ml/l which resulted in the highest rooting percentage (85.4%), root number (8.4 root/culture) with length 6.3 cm. In concentration 3.0 ml/l of Ag NPs recorded that, chlorophyll A, chlorophyll B and carotenoids increases to 1.50, 1.10 and 2.33 (mg/g). Also, occurred increase in carbohydrate content to 26.33 mg/g, the proline was enhanced and increased gradually to 3.31 mg/g and protein content to 0.77 mg/g. • Nano silver has positive effects on the growth and development of in vitro date palm cove. Hayani. • The findings indicated that the optimal medium for root formation, rooting percentage, root number and length. • Chlorophyll A, chlorophyll B, carotenoids, carbohydrate content, proline and protein content were enhanced and increased. [ABSTRACT FROM AUTHOR]