Patent Issued for Engineered transaminase polypeptides (USPTO 11788071).

The transaminase polypeptides of the present disclosure have been engineered to have one or more residue differences as compared to a previously engineered transaminase polypeptide (of amino acid sequence SEQ ID NO: 4) and associated enhanced solvent and thermal stability relative to the transaminase of SEQ ID NO: 4 and the wild-type transaminase of SEQ ID NO: 2. "In particular, the engineered transaminase polypeptides of the present disclosure have been engineered for efficient conversion of the substrate, (R)-2 SP 1 sp -(difluoromethyl)-5-methyl-2 SP 1 sp H-3-aza-1(4,2)-pyridina-2(2,3)-pyrrolacyclononaphane-4,9-dione (referred to herein as "compound (1)") to its corresponding chiral amine product compound, (5R,9S)-9-amino-2 SP 1 sp -(difluoromethyl)-5-methyl-2 SP 1 sp H-3-aza-1(4,2)-pyridina-2(2,3)-pyrrolacyclononaphan-4-one (referred to herein as "compound (2)") as shown in Scheme 2." The engineered transaminase of claim 1, wherein said engineered transaminase is a variant engineered transaminase set forth in SEQ ID NOS: 366 or 650. [Extracted from the article]