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Proteomics on malignant pleural effusions reveals ERα loss in metastatic breast cancer associates with SGK1–NDRG1 deregulation.

Authors :
Mayayo‐Peralta, Isabel
Debets, Donna O.
Prekovic, Stefan
Schuurman, Karianne
Beerthuijzen, Suzanne
Almekinders, Mathilde
Sanders, Joyce
Moelans, Cathy B.
Saleiro, Sandra
Wesseling, Jelle
van Diest, Paul J.
Henrique, Rui
Jerónimo, Carmen
Altelaar, Maarten
Zwart, Wilbert
Source :
Molecular Oncology; Jan2024, Vol. 18 Issue 1, p156-169, 14p
Publication Year :
2024

Abstract

Breast cancer (BCa) is a highly heterogeneous disease, with hormone receptor status being a key factor in patient prognostication and treatment decision‐making. The majority of primary tumours are positive for oestrogen receptor alpha (ERα), which plays a key role in tumorigenesis and disease progression, and represents the major target for treatment of BCa. However, around one‐third of patients with ERα‐positive BCa relapse and progress into the metastatic stage, with 20% of metastatic cases characterised by loss of ERα expression after endocrine treatment, known as ERα‐conversion. It remains unclear whether ERα‐converted cancers are biologically similar to bona fide ERα‐negative disease and which signalling cascades compensate for ERα loss and drive tumour progression. To better understand the biological changes that occur in metastatic BCa upon ERα loss, we performed (phospho)proteomics analysis of 47 malignant pleural effusions derived from 37 BCa patients, comparing ERα‐positive, ERα‐converted and ERα‐negative cases. Our data revealed that the loss of ERα‐dependency in this metastatic site leads to only a partial switch to an ERα‐negative molecular phenotype, with preservation of a luminal‐like proteomic landscape. Furthermore, we found evidence for decreased activity of several key kinases, including serum/glucocorticoid regulated kinase 1 (SGK1), in ERα‐converted metastases. Loss of SGK1 substrate phosphorylation may compensate for the loss of ERα‐dependency in advanced disease and exposes a potential therapeutic vulnerability that may be exploited in treating these patients. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
15747891
Volume :
18
Issue :
1
Database :
Supplemental Index
Journal :
Molecular Oncology
Publication Type :
Academic Journal
Accession number :
174603762
Full Text :
https://doi.org/10.1002/1878-0261.13540