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A comparison of osteogenic effect of newly manufactured calcium silicate-based sealers in vitro.

A comparison of osteogenic effect of newly manufactured calcium silicate-based sealers in vitro.

Authors :
Kkot-Byeol BAE
Yoorina CHOI
Bin-Na LEE
Hoon-Sang CHANG
In-Nam HWANG
Won-Mann OH
Yun-Chan HWANG
Source :
Dental Materials Journal; 2023, Vol. 42 Issue 6, p860-867, 8p
Publication Year :
2023

Abstract

This study aimed to assess the effect of different calcium silicate-based root canal sealers (CSRS) on osteogenic effect in human periodontal ligament cells (hPDLCs). hPDLCs were cultured in a medium containing extract of 5 types of CSRS. The specimens were assessed by the cell cytotoxicity test, alkaline phosphatase staining, alizarin red S staining, quantitative real-time PCR, Western blot analysis, and enzyme-linked immunosorbent assay. The diluted concentrations of extracted solutions had no significant effect on the viability of hPDLCs. There was a statistically significant difference in the mRNA expression level of bone sialoprotein (BSP), osteocalcin (OCN), and runt-related transcription factor 2 (RUNX2) among some groups. The protein expressions of BSP, OCN, and RUNX2 were significantly higher in some groups compared to the control group. The CSRS did not interfere with the osteogenic differentiation of hPDLCs, compared to the control group. CSRS are shown to have biocompatibility and osteogenic differentiation effect on hPDLCs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
02874547
Volume :
42
Issue :
6
Database :
Supplemental Index
Journal :
Dental Materials Journal
Publication Type :
Academic Journal
Accession number :
175512628
Full Text :
https://doi.org/10.4012/dmj.2023-048