Rapid Human Oogonia-like Cell Specification via Combinatorial TF-Directed Differentiation (Updated February 20, 2024).

A recent preprint abstract discusses the generation of germline cell types from human induced pluripotent stem cells (hiPSCs) for in vitro gametogenesis. The study focuses on the use of transcription factors (TFs) to directly differentiate hiPSCs into specific cell types, including ovarian cell types. The researchers identified several TFs that drive the formation of induced oogonia-like cells (iOLCs) and human primordial germ cell-like cells (hPGCLCs) from hiPSCs. The study also demonstrates a simple culture condition for the formation of iOLCs and a method for expanding and maintaining their cell identity in vitro. These findings contribute to the understanding of human germ cell specification and provide new tools for in vitro oogenesis research. [Extracted from the article]