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Periostin regulates integrin expression in gingival epithelial cells.

Periostin regulates integrin expression in gingival epithelial cells.

Authors :
Hirata, Reika
Iwata, Tomoyuki
Fujita, Tsuyoshi
Nagahara, Takayoshi
Matsuda, Shinji
Sasaki, Shinya
Taniguchi, Yuri
Hamamoto, Yuta
Ouhara, Kazuhisa
Kudo, Yasusei
Kurihara, Hidemi
Mizuno, Noriyoshi
Source :
Journal of Oral Biosciences; Mar2024, Vol. 66 Issue 1, p170-178, 9p
Publication Year :
2024

Abstract

Human gingival epithelial cells (HGECs) function as a mechanical barrier against invasion by pathogenic organisms through epithelial cell–cell junction complexes, which are complex components of integrin. Integrins play an important role in the protective functions of HGECs. Human periodontal ligament (HPL) cells regulate periodontal homeostasis. However, periodontitis results in the loss of HPL cells. Therefore, as replenishment, HPL cells or mesenchymal stem cells (MSCs) can be transplanted. Herein, HPL cells and MSCs were used to elucidate the regulatory mechanisms of HGECs, assuming periodontal tissue homeostasis. Human gingival fibroblasts (HGFs), HGECs, HPL cells, and MSCs were cultured, and the conditioned medium was collected. With or without silencing periostin mRNA, HGECs were cultured under normal conditions or in a conditioned medium. Integrin and periostin mRNA expression was determined using real-time polymerase chain reaction. Integrin protein expression was analyzed using flow cytometry, and periostin protein expression was determined via western blotting. The conditioned medium affected integrin expression in HGECs. Higher expression of periostin was observed in MSCs and HPL cells than in HGFs. The conditioned medium that contained periostin protein regulated integrin expression in HGECs. After silencing periostin in MSCs and HPL cells, periostin protein was not detected in the conditioned medium, and integrin expression in HGECs remained unaffected. Integrins in HGECs are regulated by periostin secreted from HPL cells and MSCs. This result suggests that periostin maintains gingival cell adhesion and regulates bacterial invasion/infection. Therefore, the functional regulation of periostin-secreting cells is important in preventing periodontitis. • Integrins on HGEC are regulated by humoral factor from HPL cells and MSCs. • Periostin is included in humoral factors from HPL cells and MSCs. • Periostin maintains gingival cell adhesion via the regulation of integrins. • Periostin can regulate bacterial invasion. • Regulation of periostin-secreting cells is important to prevent periodontitis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13490079
Volume :
66
Issue :
1
Database :
Supplemental Index
Journal :
Journal of Oral Biosciences
Publication Type :
Academic Journal
Accession number :
175834601
Full Text :
https://doi.org/10.1016/j.job.2023.11.009