HPLC-Q-TOF-MS ANALYSIS OF PHENOLIC COMPOUNDS, IN VITRO BIOLOGICAL ACTIVITIES AND IN VIVO ACUTE TOXICITY EVALUATION OF Ocimum Basilicum L.

Background: Sweet Basil (Ocimum basilicum L.) is regarded as a significant plant. They include a wide variety of bioactive substances, particularly phenolic substances which contribute to the plant's alleged health advantages. Aim: The objective of this study was to identify and characterize the phenolic profile of sweet basil using HPLC-Q-TOF-MS in order to evaluate its anti-inflammatory and antioxidant properties. Methods: By using standard protocols, Bioactive molecules were extracted and qualitative tests of phytochemicals were also released as well as quantitative analyses of total phenols, total flavonoids, and total hydrolysable tannins. Using HPLCQ-TOF-MS, phenolic compounds can be identified. Antioxidant and anti-inflammatory activities were studied by DPPH, FRAP, RBC membrane stabilization and BSA anti denaturation assays. Results: 30 phenolic compounds in this plant were identified by the HPLC-Q-TOF-MS analysis. Moreover, the TPC, TFC, and THTC of sweet basil was systematically assessed, and then its antioxidant and anti-inflammatory properties were evaluated using the DPPH, FRAP, RBC membrane stabilization, and BSA anti denaturation assays. Significant anti-inflammatory and antioxidant effects were also seen in the plant extracts with high phenolic, flavonoid, and tannin content. Conclusion: The results demonstrate the value of sweet basil (Ocimum basilicum L.) which may be used in the food and health industries as promising sources of phenolic chemicals. [ABSTRACT FROM AUTHOR]