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Development of an Integrated Disposable Device for SARSCoV-2 Nucleic Acid Extraction and Detection.
- Source :
- Biomedical & Environmental Sciences; Jun2024, Vol. 37 Issue 6, p639-646, 8p
- Publication Year :
- 2024
-
Abstract
- To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We designed, developed, and manufactured an integrated disposable device for SARS-CoV-2 nucleic acid extraction and detection. The precision of the liquid transfer and temperature control was tested. A comparison between our device and a commercial kit for SARS-Cov-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). The entire process, from SARS-CoV-2 nucleic acid extraction to amplification, was evaluated. The precision of the syringe transfer volume was 19.2 ± 1.9 μL (set value was 20), 32.2 ± 1.6 (set value was 30), and 57.2 ± 3.5 (set value was 60). Temperature control in the amplification tube was measured at 60.0 ± 0.0 °C (set value was 60) and 95.1 ± 0.2 °C (set value was 95) respectively. SARS-Cov- 2 nucleic acid extraction yield through the device was 7.10 × 10<superscript>6</superscript> copies/mL, while a commercial kit yielded 2.98 × 10<superscript>6</superscript> copies/mL. The mean time to complete the entire assay, from SARS-CoV-2 nucleic acid extraction to amplification detection, was 36 min and 45 s. The detection limit for SARS-CoV-2 nucleic acid was 250 copies/mL. The integrated disposable devices may be used for SARS-CoV-2 Point-of-Care test (POCT). [ABSTRACT FROM AUTHOR]
- Subjects :
- SARS-CoV-2
REVERSE transcriptase polymerase chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 08953988
- Volume :
- 37
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Biomedical & Environmental Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 178596137
- Full Text :
- https://doi.org/10.3967/bes2024.070