Investigation of antioxidant, antityrosinase, anticollagenase and cytotoxic effects of some Asphodelus species as potential dermocosmetic agent.

Various studies have reported the antimicrobial, antifungal, and antioxidant effects of Asphodelus species. These species are used in the treatment of various skin-related issues such as acne, abscesses, psoriasis, burns, alopecia, wound healing, and eczema in folk medicine. The purpose of the present study is to determine the antioxidant, antityrosinase, anticollagenase and cytotoxic effects of Asphodelus aestivus and Asphodelus fistulosus as potential dermocosmetic agent, as well as the phenolic substance profiles of the species by Reversed phase high performance liquid chromatography (RP-HPLC). Antioxidant capacity of the species was determined by total phenolic and flavonoid content determination, cupric ion reducing power (CUPRAC), ferric ion reducing power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assays. Enzyme inhibition studies were conducted spectrophotometrically. The cytotoxic activity was analyzed by the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay. As a result, it was determined that the methanolic extracts obtained from the species showed strong antioxidant activity. Based on the CUPRAC and DPPH results, A. aestivus was found to be more effective while according to the FRAP results, A. fistulosus exhibited greater effectiveness. A. fistulosus was discovered to be abundant in phenolic substances, while A. aestivus was found to be richer in flavonoids. It has also been observed that the methanolic extracts have a high inhibitory effect on collagenase and tyrosinase enzymes. It is believed that the species can be used as a potential resource in the treatment of skin aging and hyperpigmentation problems in the dermocosmetic industry. It is thought that the data obtained in the study will lead to further studies. [Display omitted] • The methanolic extracts obtained from A. aestivus and A. fistulosus showed strong antioxidant activity. • Tyrosinase and collagenase enzyme inhibition activities of the extracts are significant. • It was observed that the methanol extract of A. aestivus had a higher inhibitory effect on enzymes. • It was found that the extracts did not affect cell viability even at maximum concentration. • In HPLC studies, it was determined that the species contained p-hydroxy benzoic acid, syringaldehyde, benzoic acid, quercetin, vanillic acid and sinapic acid as phenolic compounds. [ABSTRACT FROM AUTHOR]