Elevating biotransamiantion of pineapple peel-derived furfural into furfurylamine by recombinant Escherichia coli carrying pyruvate decarboxylase and mutant ω-transaminase with low loading of amine donor.

Furfurylamine is a versatile compound used in perfumes, polymers, pharmaceuticals, and agrochemicals. To address the costly and inefficient furfural bioamination process, a strategy combining ω-transaminase with other enzymes was applied to construct a multi-enzyme system. Pyruvate decarboxylase (PDC) might transform amination byproducts into acetaldehyde, promoting the forward bioreaction. E. coli 20PDC was created by co-expressing PDC and mutated ω-transaminase (HN), which could be applied to efficiently aminate furfural, vanillin and 5-hydroxymethylfurfural into furfuryalmine, vanillylamine, and 5-hydroxymethyl-2-furfurylamine, respectively. Pineapple peel can be used as a suitable feedstock for production of furfural and furfurylamine. A chemobiological cascade reaction was built to transform pineapple peel into furan-based amines in deep eutectic solvent ChCl: p A-H 2 O. In ChCl: p A (10 wt%) at 170 °C, pineapple peel was converted to furfural within 10 min (65.2% yield, based on xylan), and the pineapple peel-derived furfural was resoundingly converted to furfurylamine at 35 °C with D -alanine (D -alanine:substrate = 2:1) in ChCl: p A (20 wt%, pH 7.5). This dual-enzyme co-expression strategy reduced the amine donor loading and realized the effectual bioamination from biomass-derived aldehydes. To sum up, the strategy for synthesis of FAM from pineapple peel by tandem chemocatalysis and biocatalysis in ChCl: p A-H 2 O holds significant promising in the valorization of biomass into valuable furans. [Display omitted] • Furfurylamine (FAM) was prepared from pineapple peel via chemobiocatalytic approach. • ChCl: p A–H 2 O catalyzed pineapple peel to FUR (65.2% yield) at 170 °C for 10 min. • E. coli 20PDC was created by co-expressing PDC and mutated ω-transaminase (HN). • A small amount of D -alanine was required for the bioamination of FUR into FAM. • The FAM yield was 0.47 g FAM/(g xylan in pineapple peel). [ABSTRACT FROM AUTHOR]