The ISPpu9 insertion sequence of Pseudomonas putida KT2440 generates various circular intermediates enabling modular transposition.

The article discusses the ISPpu9 insertion sequence found in Pseudomonas putida KT2440, which generates circular intermediates enabling modular transposition. This insertion sequence contains seven copies in the KT2440 strain, with five copies including a small RNA called ssr9. The study found that circular intermediates containing different combinations of the transposase and ssr9 were detected, shedding light on the distinct modules present in the KT2440 chromosome. The research suggests that the formation of hybrid promoters in these circular intermediates may depend on the strength of the native transposase promoter. [Extracted from the article]