Back to Search
Start Over
C-KIT expression in ductal carcinoma in situ of the breast: co-expression with HER-2/neu.
- Source :
- Human Pathology; Feb2006, Vol. 37 Issue 2, p205-211, 7p
- Publication Year :
- 2006
-
Abstract
- Summary: The proto-oncogene c-KIT (CD117) is highly expressed in normal breast epithelium and is decreased in invasive breast cancer. In this study, we analyzed the protein expression and the mutational status of c-KIT in ductal carcinoma in situ (DCIS) of the breast and correlated these findings with nuclear grade, architectural pattern, and expression of HER-2, estrogen receptor (ER)–α, and progesterone receptor (PR). C-KIT, HER-2, ER, and PR expression were analyzed immunohistochemically in 106 cases of paraffin-embedded DCIS (85 pure DCIS and 21 DCIS with concurrent carcinoma). Direct sequencing of exons 9 and 11 of the c-KIT gene was performed to analyze the hot spot mutational regions in representative cases. C-KIT expression was found in 55 (52.8%) of all DCIS, correlating with high nuclear grade (P < .0001), comedonecrosis (P < .0001), and solid growth pattern (P = .001). Furthermore, c-KIT expression was strongly associated with HER-2 positivity (P < .0001) and was significantly lower in ER- or PR-positive cases (P = .001 and P = .006, respectively). C-KIT expression alone or co-expression with HER-2 in pure DCIS did not differ significantly from DCIS with invasive component (P = .09). Mutational analysis in 6 c-KIT–positive DCIS revealed no activating mutations in exons 9 or 11. Our findings suggest that the expression of c-KIT protein might define a subset of poorly differentiated, HER-2–positive DCIS with decreased expression of steroid hormone receptors, comedonecrosis, and a solid growth pattern. The implications of c-KIT and HER-2 co-expression for breast carcinogenesis should be further evaluated. [Copyright &y& Elsevier]
- Subjects :
- BREAST
CORPUS luteum
PROGESTATIONAL hormones
DRUG receptors
Subjects
Details
- Language :
- English
- ISSN :
- 00468177
- Volume :
- 37
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Human Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 22636392
- Full Text :
- https://doi.org/10.1016/j.humpath.2005.10.015