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Immunoassay Using Microfluid Filters Constructed by Deep X-Ray Lithography.

Authors :
Matsui, Katsuhiro
Kawaji, Isao
Utsumi, Yuichi
Ukita, Yoshiaki
Asano, Toshifumi
Takeo, Masahiro
Kato, Dai-ichiro
Negoro, Seiji
Source :
Bioscience, Biotechnology & Biochemistry; Dec2007, Vol. 71 Issue 12, p3098-3101, 4p, 1 Diagram, 2 Graphs
Publication Year :
2007

Abstract

The article evaluates the effectiveness of a microfluidic filter for biochemical reactions by performing a sandwich enzyme-linked immunosorbent assay (ELISA) of mouse immunoglobulin G IgG). According to the authors, microfluidic filters were fabricated by deep X-ray lithography using synchrotron radiation. They add that mouse IgG could be quantitatively detected in the range of 0-100 mg/ml, showing the applicability of vertical microfluidic operation to the immunoassay.

Details

Language :
English
ISSN :
09168451
Volume :
71
Issue :
12
Database :
Supplemental Index
Journal :
Bioscience, Biotechnology & Biochemistry
Publication Type :
Academic Journal
Accession number :
31793066
Full Text :
https://doi.org/10.1271/bbb.70415