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Potential role of antibodies against cardiac Kv channel-interacting protein 2 in dilated cardiomyopathy.
- Source :
- American Heart Journal; Jul2008, Vol. 156 Issue 1, p92-99.e2, 0p
- Publication Year :
- 2008
-
Abstract
- Background: Growing evidence suggests participation of autoimmune mechanisms in the pathogenesis of dilated cardiomyopathy (DCM). Methods: Patients with heart failure (left ventricular ejection fraction ≤50%) due to DCM (n = 98) or ischemic cardiomyopathy (ICM, n = 49) and controls with normal left ventricular function (n = 98) were included. Immunoglobulin G antibodies were purified from plasma by affinity chromatography and analyzed by surface plasmon resonance analysis. We analyzed the distribution of autoantibodies against Kv channel-interacting protein (KChIP) 2.6, cardiac troponin I (cTnI), and the β1-adrenergic receptor (second extracellular loop, cardiac β1-adrenergic receptor [SEL-β1-AR])—two other known autoantibodies involved in heart failure. Effects of antibodies against KChIP2 on cell death of isolated rat cardiomyocytes were assessed by flow cytometry. Results: We detected autoantibodies against KChIP2.6 in 14.3% (P < .015 vs controls, P = .286 vs ICM) of the DCM samples, in 8.2% of the ICM samples (P = .304 vs controls), and in 4.1% of the control samples. Virus persistence was significantly associated with detection of autoantibodies against KChIP2.6 in DCM patients (P = .025). Antibodies against SEL-β1-AR were more frequent in DCM samples (34.7%, P < .001 vs controls, P = .02 vs ICM) and ICM samples (16.3%, P = .083 vs control) than in control samples (7.1%). Antibodies against cTnI were more frequent in DCM samples (20.4%, P < .001 vs controls, P = .769 vs ICM) and in ICM samples (18.4%, P < .01 vs controls) than in control samples (4.1%). Antibodies against rat KChIP2 enhanced cell death in isolated rat cardiomyocytes. Immunofluorescence indicated cell surface expression of KChIP2. Conclusions: Autoantibodies against KChIP2.6, SEL-β1-AR, and cTnI appear to be associated with DCM. Antibodies against KChIP2 may enhance cell death of rat cardiomyocytes. [Copyright &y& Elsevier]
- Subjects :
- IMMUNOGLOBULINS
CARDIOMYOPATHIES
CHROMATOGRAPHIC analysis
SURFACE plasmon resonance
Subjects
Details
- Language :
- English
- ISSN :
- 00028703
- Volume :
- 156
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- American Heart Journal
- Publication Type :
- Academic Journal
- Accession number :
- 32845829
- Full Text :
- https://doi.org/10.1016/j.ahj.2008.02.015