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Application of Donor Lymphocytes Expressing a Suicide Gene for Early GVL Induction and Later Control of GVH Reactions After Bone-Marrow Transplantation.
- Source :
- Adoptive Immunotherapy: Methods & Protocols; 2005, p475-486, 12p
- Publication Year :
- 2005
-
Abstract
- Allogeneic hematopoietic stem cell transplantation (allo-SCT) is the treatment of choice for many malignant diseases. It is recognized that the curative potential of allo-SCT relates closely to the immune advantage conferred by allogeneic T-lymphocytes. In allo-SCT donor T-lymphocytes favor engraftment, provide early immune reconstitution, and fight the underlying malignancy-the so-called graft-vs-leukemia (GVL) effect. These benefits are counterbalanced by the occurrence of a life-threatening disease: graft-vs-host disease (GVHD). A suicide gene encodes a protein able to convert a nontoxic prodrug into a toxic product. Therefore, cells expressing the suicide gene become selectively sensitive to the prodrug. The transfer of a suicide gene into donor lymphocytes could allow, upon administration of the prodrug, the in vivo selective elimination of transduced lymphocytes, resulting in the switch-off of GVHD, thus allowing full exploitation of the curative potential of donor T-lymphocytes in the context of allo-SCT. In this chapter the rationale, the materials, and the methods of the suicide-gene strategy with human T-lymphocytes are described. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISBNs :
- 9781588294067
- Database :
- Supplemental Index
- Journal :
- Adoptive Immunotherapy: Methods & Protocols
- Publication Type :
- Book
- Accession number :
- 33085268
- Full Text :
- https://doi.org/10.1385/1-59259-862-5:475