Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression.

Ligation-mediated polymerase chain reaction (LM-PCR) is used for high-resolution genomic footprinting. This technique was originally developed to study in vivo protein-deoxyribonucleic acid (DNA) interactions at regions of genes important for transcriptional regulation. Successful analysis of in vivo occupancy of gene regulatory elements by LM-PCR largely depends on the strategy and quality of reagents. The experimental outline for LM-PCR reaction is shown in Fig. 1. For a through understanding of this procedure and other alternative options, see Ausubel et al. (1). [ABSTRACT FROM AUTHOR]