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Construction of Double Right-Border Binary Vector Carrying Non-Host Gene Rxo1 Resistant to Bacterial Leaf Streak of Rice.
- Source :
- Rice Science; Sep2008, Vol. 15 Issue 3, p243-246, 4p
- Publication Year :
- 2008
-
Abstract
- Abstract: Rxo1 cloned from maize is a non-host gene resistant to bacterial leaf streak of rice. pCAMBIA1305-1 with Rxo1 was digested with Sca I and NgoM IV and the double right-border binary vector pMNDRBBin6 was digested with Hpa I and Xma I. pMNDRBBin6 carrying the gene Rxo1 was acquired by ligation of blunt-end and cohesive end. The results of PCR, restriction enzyme analysis and sequencing indicated that the Rxo1 gene had been cloned into pMNDRBBin6. This double right-border binary vector, named as pMNDRBBin6-Rxo1, will play a role in breeding marker-free plants resistant to bacterial leaf streak of rice by genetic transformation. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 16726308
- Volume :
- 15
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Rice Science
- Publication Type :
- Academic Journal
- Accession number :
- 34982445
- Full Text :
- https://doi.org/10.1016/S1672-6308(08)60048-7