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Anti-tumor Immunity of Newcastle Disease Virus HN Protein is Influenced by Differential Subcellular Targeting.
- Source :
- Chinese Journal of Lung Cancer; Aug2010, Vol. 13 Issue 8, p773-776, 4p, 1 Chart, 2 Graphs
- Publication Year :
- 2010
-
Abstract
- Background and objective Hemagglutinin-neuraminidase (HN) protein of newcastle disease virus is an important immunogen for oncolysis. We designed three different expression plasmids encoding the HN protein targeted to different subcellular compartments: cytoplasmic (Cy-HN), secreted (Sc-HN) and membrane-anchored (M-HN). On the basis of anti-tumor effect in vitro, the aim of this study is to investigate the anti-tumor immunity effect of HN protein in vivo. Methods In the present study, we developed a mouse model in order to evaluate the anti-tumor effect of the intratumorally injected modified HN proteins and the anti-tumor immunity by lymphocyte proliferative response and CTL activity test. Results Although all three DNA constructs elicited an immune response, tumor-bearing mice intratumorally injected with M-HN demonstrated a significantly better anti-tumor effect than those injected with Cy-HN or Sc-HN (Day 18: P=0.022; Day 21: P<0.01). It also showed that this anti-tumor effect was mediated by higher lymphocyte proliferative response and CTL activity in mice intratumorally injected with M-HN [M-HN vs Cy-HN, P=0.019; M-HN vs Sc-HN, P=0.043; M-HN vs pcDNA3.1(+), P<0.01]. Conclusion The anti-tumor immunity of Newcastle disease virus HN protein is influenced by differential subcellular targeting. The membrane-anchored form of the HN protein appears to be an ideal candidate to improve the specific cellular immunity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 10093419
- Volume :
- 13
- Issue :
- 8
- Database :
- Supplemental Index
- Journal :
- Chinese Journal of Lung Cancer
- Publication Type :
- Academic Journal
- Accession number :
- 61290001
- Full Text :
- https://doi.org/10.3779/j.issn.1009-3419.2010.08.04