Direct laser printing of oligonucleotides for the fabrication of a label-free biosensor.

Abstract: This work presents the direct laser printing of thiol-modified oligonucleotides onto the surface of 3- glycidoxypropyltrimethoxysilane (GOPTS)-functionalized LTO on Si planar substrates and onto (GOPTS)- functionalized capacitive sensor membranes. The (GOPTS)-functionalized planar LTO on Si surfaces have been used to select the optimum deposition conditions and to confirm the ability of the oligonucleotides to recognize their complementary targets in solution. This reaction was confirmed by fluorescence microscopy. The purpose of those experiments was the fabrication of a label-free biosensor. [Copyright &y& Elsevier]