Back to Search
Start Over
Optimization of Multiplate® whole blood platelet aggregometry in the Beagle dog and Wistar rat for ex vivo drug toxicity testing.
- Source :
- Experimental & Toxicologic Pathology; Jul2013, Vol. 65 Issue 5, p637-644, 8p
- Publication Year :
- 2013
-
Abstract
- Abstract: This study was performed to optimize and standardize the use of the Multiplate<superscript>®</superscript> whole blood impedance aggregometer in the Beagle dog and Wistar rat for use in a research laboratory environment. The anticoagulants citrate, heparin and hirudin were compared and platelet aggregation responses to ADP, collagen, arachidonic acid and Par-4 agonist were evaluated to determine their half maximal effective concentrations (EC<subscript>50</subscript>) in blood containing low concentrations of a drug solvent (0.1% DMSO). The results indicate that citrate anticoagulation is not suitable for Multiplate<superscript>®</superscript> whole blood aggregometry because of the presence of spontaneous aggregation. ADP and collagen were found to be appropriate agonists for both species, whereas in the Beagle dog Par-4 agonist failed to induce aggregation and arachidonic acid induced platelet aggregation showed a high interindividual variability. The agonists EC<subscript>50</subscript> calculated in hirudin blood were 2.70μM ADP, 0.85μg/ml collagen, 0.03mM arachidonic acid and 165.7μM Par-4 agonist in the Wistar rat, and 0.95μM ADP and 0.23μg/ml collagen in the Beagle dog. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09402993
- Volume :
- 65
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- Experimental & Toxicologic Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 87824729
- Full Text :
- https://doi.org/10.1016/j.etp.2012.07.003