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In vitro inhibition of porcine reproductive and respiratory syndrome virus replication by short hairpin RNA targeting to 5' untraslated region.

Authors :
HUANG Juan
JIANG Ping
Source :
Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao; oct2013, Vol. 33 Issue 10, p1514-1518, 5p
Publication Year :
2013

Abstract

The article discusses a study of in vitro inhibition of porcine reproductive and respiratory syndrome virus (PRRSV) replication by short hairpin ribonucleic acid (shRNA) targeting to 5' untraslated region (UTR). RNA interfering efficiency was assayed by fluorocyte and cytopathic effect observation, indirect immunofluorescence assay (IFA) and real time PCR. It states that shRNA targeting to 5' UTR could inhibit PRRSV replication that is essential for infectivity of porcine reproductive.

Details

Language :
Chinese
ISSN :
10054545
Volume :
33
Issue :
10
Database :
Supplemental Index
Journal :
Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao
Publication Type :
Academic Journal
Accession number :
93454369