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An In Vitro Genotoxicity Study of Biphasic Calcium Phosphate on Ames Test.
- Source :
- International Medical Journal; Feb2014, Vol. 21 Issue 1, p34-37, 4p, 2 Charts, 2 Graphs
- Publication Year :
- 2014
-
Abstract
- Background: Biphasic Calcium Phosphate (BCP) is being engineered as a bone substitute material that can be widely used in maxillofacial, dentistry and orthopaedic applications. The objective of this in vitro study was to evaluate the genotoxicity of BCP using Bacterial Reverse Mutation Assay (Ames Test). Methods: This was a descriptive experimental study performed on Salmonella typhimurium TA1538 strain using pre-incubation method in the presence and absence of an exogenous metabolic activation system. The bacteria were incubated for 48 hours at 37 ± 0.5°C before the colony growth or revertant colonies were counted. Data obtained were analyzed by using non-statistical method. Results: The investigation of the genotoxic reaction on the test material revealed that the numbers of revertant colonies in strain with and without S9 mix were less than twice of the negative control for all tested concentration. Conclusion: The results of the tests showed that BCP was considered to be non-mutagenic under the present test condition. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13412051
- Volume :
- 21
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- International Medical Journal
- Publication Type :
- Academic Journal
- Accession number :
- 95523236