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Determination of Chlorpromazine and Its Sulfoxide and 7-Hydroxy Metabolites by Ion-Pair High Pressure Liquid Chromatography
- Source :
- Analytical Letters; January 1981, Vol. 14 Issue: 10 p741-761, 21p
- Publication Year :
- 1981
-
Abstract
- An ion-pair HPLC approach with ordinary silica has been applied, with detection by ultraviolet absorption, to the assay of plasma for chlorpromazine and its sulfoxide on the one hand, and for 7-hydroxychlorpromazine (an active metabolite) on the other hand. The respective sample-preparation procedures entail extraction of the plasma with heptane at strongly alkaline pH, or else with diethyl ether at a less alkaline pH and with ensuing back-extraction and re-extraction. For each of the compounds, levels as low as 10 ng.ml-1 are measurable. The conditions adopted are such that specificity and reproducibility are satisfactory although chlorpromazine and its various metabolites, especially 7-hydroxychlorpromazine, are chemically unstable and, moreover, are readily lost onto glass. With the unorthodox separation system adopted, adsorption rather than partition appears to be the dominant mechanism.
Details
- Language :
- English
- ISSN :
- 00032719 and 1532236X
- Volume :
- 14
- Issue :
- 10
- Database :
- Supplemental Index
- Journal :
- Analytical Letters
- Publication Type :
- Periodical
- Accession number :
- ejs11087126
- Full Text :
- https://doi.org/10.1080/00032718108055479