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The trkBtyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3
- Source :
- Cell; July 1991, Vol. 66 Issue: 2 p395-403, 9p
- Publication Year :
- 1991
-
Abstract
- trkB is a tyrosine protein kinase gene highly related to trk, a proto-oncogene that encodes a receptor for nerve growth factor (NGF) and neurotrophin-3 (NT-3). trkB expression is confined to structures of the central and peripheral nervous systems, suggesting it also encodes a receptor for neurotrophic factors. Here we show that brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of gp145trkB, one of the receptors encoded by trkB. BDNF and NT-3 can induce DNA synthesis in quiescent NIH 3T3 cells that express gp145trkB. Cotransfection of plasmids encoding gp145trkBand BDNF or NT-3 leads to transformation of recipient NIH 3T3 cells. In these assays, BDNF elicits a response at least two orders of magnitude higher than NT-3. Finally, 125I-NT-3 binds to NIH 3T3 cells expressing gp145trkB; binding can be competed by NT-3 and BDNF but not by NGF. These findings indicate that gp145trkBmay function as a neurotrophic receptor for BDNF and NT-3.
Details
- Language :
- English
- ISSN :
- 00928674 and 10974172
- Volume :
- 66
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Cell
- Publication Type :
- Periodical
- Accession number :
- ejs11466825
- Full Text :
- https://doi.org/10.1016/0092-8674(91)90628-C