Immobilization of DNA on Self-Assembled Monolayer

AbstractWe attempted the immobilization of DNA onto self-assembled monolayer (SAM) which contains the intercalated for DNA. Anthryl moieties were attached to the terminal OH groups in SAM of 1-Mercapt-11-undecanol (MU-SAM) by esterification. In the case of the SAM containing anthryl moieties (Anth-SAM), surface plasmon resonance measurement indicates that the adsorption of DNA onto the SAM was observed in an aqueous DNA solution, and the DNA remained on the surface even after rinsing. Although the adsorption of DNA onto MU-SAM was also observed, little DNA remained on the SAM after rinsing. These results indicate that the anthracene which was attached to the SAM can interact with DNA because the binding ability was increased by introduction of anthracene moieties to the SAM.