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Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magenetic particles and fully automated system
- Source :
- Applied Biochemistry and Biotechnology; March 2001, Vol. 91 Issue: 1-9 p109-116, 8p
- Publication Year :
- 2001
-
Abstract
- Abstract: A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contians a reaction station, tip rack, and an eight-tip pipettor that is alble to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
Details
- Language :
- English
- ISSN :
- 02732289 and 15590291
- Volume :
- 91
- Issue :
- 1-9
- Database :
- Supplemental Index
- Journal :
- Applied Biochemistry and Biotechnology
- Publication Type :
- Periodical
- Accession number :
- ejs12749569
- Full Text :
- https://doi.org/10.1385/ABAB:91-93:1-9:109