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Monoclonal antibody 57B stains tumor tissues that express gene <TOGGLE>MAGE-A4</TOGGLE>
- Source :
- International Journal of Cancer; 15 June 2000, Vol. 86 Issue: 6 p835-841, 7p
- Publication Year :
- 2000
-
Abstract
- Monoclonal antibody (Mab) 57B, which was raised against a recombinant MAGE-A3 protein, was tested for its ability to stain cells expressing various members of the MAGE-A gene family. COS-7 cells transfected with cDNAs encoding MAGE-A1, A2, A3, A4, A6, or A12 were stained, whereas those transfected with MAGE-A8, A9, A10, or A11 cDNAs were not. However, in tissue sections, we observed a different pattern of staining: the antibody effectively stained the tumors that expressed MAGE-A4 and only these tumors, regardless of the expression of the other MAGE-A genes. It seems, therefore, that at the level of MAGE gene expression found in tumors, a level clearly lower than that observed in transfected COS cells, only the MAGE-A4 protein can be reliably detected. We conclude that the 57B Mab should be useful for tumor diagnosis related to therapeutic vaccination involving MAGE-A4. Int. J. Cancer 86:835841, 2000. © 2000 Wiley-Liss, Inc.
Details
- Language :
- English
- ISSN :
- 00207136 and 10970215
- Volume :
- 86
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- International Journal of Cancer
- Publication Type :
- Periodical
- Accession number :
- ejs1284536
- Full Text :
- https://doi.org/10.1002/(SICI)1097-0215(20000615)86:6<835::AID-IJC12>3.0.CO;2-N