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NYD-SP15 : A Novel Gene Potentially Involved in Regulating Testicular Development and Spermatogenesis
- Source :
- Biochemical Genetics; August 2006, Vol. 44 Issue: 7-8 p405-419, 15p
- Publication Year :
- 2006
-
Abstract
- By hybridizing human adult testis cDNA microarrays with human adult and embryo testis cDNA probes, we identified a novel human testis gene, NYD-SP15. NYD-SP15 expression was 3.26-fold higher in adult than in fetal testis; however, there was almost no NYD-SP15 expression in the sperm. NYD-SP15 comprises 3364 base pairs, including a 1545 bp open reading frame encoding a 514 amino acid protein possessing 89% sequence identity with the mouse testis homologous protein. NYD-SP15 is located on human chromosome 13q14.2. The deduced structure of the protein contains two dCMP_cyt_deam domains, indicating a potential functional role for zinc ion binding. The gene is expressed variably in a wide range of tissues, with high expression levels in the testis. Sequence analysis revealed that NYD-SP15 is not a highly conserved protein, with its distribution in high-level species such as vertebrates including Homo, Mus, Rattus, and Canis. The results of semiquantitative polymerase chain reaction in mouse testis representing different developmental stages indicate that NYD-SP15 expression was developmentally regulated. These results suggest the putative NYD-SP15 protein may play an important role in testicular development and spermatogenesis and may be an important factor governing male infertility.
Details
- Language :
- English
- ISSN :
- 00062928 and 15734927
- Volume :
- 44
- Issue :
- 7-8
- Database :
- Supplemental Index
- Journal :
- Biochemical Genetics
- Publication Type :
- Periodical
- Accession number :
- ejs14936948
- Full Text :
- https://doi.org/10.1007/s10528-006-9038-x