Production of endopolygalacturonase fromRhizopus nigricansrelated to the evolution of growth substrate

Endopolygalacturonases production byRhizopus nigricansis studied. The enzymatic level present during the early growth stages depolymerized fully the substrate and afterwards the growth of the fungi, with the parallel production of endopolygalacturonase, began. The maximum level of enzymatic activity was 18 U/mL and was reached six days after the maximun metabolic activity of the fungi. The production of endopolygalacturonase finished when the carbon source was exhausted.