Effects of temperature on growth and intracellular proteins of Norwegian Typhula ishikariensis isolates

Growth of two groups of strains (group I and III) of the snow mould fungus, Typhula ishikariensis from northern Norway, were studied in agar and liquid culture. All strains could grow from—5 to 15 (group III) or 20°C (group I) and the optimum temperatures for growth were 4 or 10°C, respectively. The group III strains showed irregular growth as the hypal extensions were inhibited at 15°C. Different temperature two-dimensional electrophoresis revealed that in the group III strains some intracellular proteins were modified at the highest temperature. One of the reasons that group III strains did not grow or showed irregular growth at 20 or 15°C, was probably due to protein aggregation or dissociation and thereby loss of vital properties of intracellular proteins.