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Effects of Mercury on Dictyostelium discoideum: Proteomics Reveals the Molecular Mechanisms of Physiological Adaptation and Toxicity
- Source :
- Journal of Proteome Research; 20240101, Issue: Preprints
- Publication Year :
- 2024
-
Abstract
- Dictyostelium discoideumamoebae were exposed to Hg 2 μM corresponding to a sublethal concentration and Hg 10 μM with the first effects on mortality and replication rate. A total of 900 spots were visualized by 2-DE electrophoresis. Two-hundred fifty single proteins were identified by mass spectrometry. Low Hg concentration (2 μM) treatment induced up-regulation of 13 spots, mainly involved in oxidative stress response/detoxification, oxidoreductase activity, and metabolic processes. High Hg concentration (10 μM) treatment showed a different PES with 12 proteins downregulated and only two up-regulated, mainly involved in cellular metabolic processes, metal ion binding, and transferase activity. The analyses for the carbonylation show no changes after 2 μM Hg2+treatment and 13 differentially carbonylated proteins after 10 μM Hg2+involved in a broad range of cellular processes. Our findings provide insight into the mechanisms of physiological adaptation and toxicity to a low and an high mercury concentration, respectively, of Dictyostelium amoebae.
Details
- Language :
- English
- ISSN :
- 15353893 and 15353907
- Issue :
- Preprints
- Database :
- Supplemental Index
- Journal :
- Journal of Proteome Research
- Publication Type :
- Periodical
- Accession number :
- ejs21200996
- Full Text :
- https://doi.org/10.1021/pr900914t