Back to Search
Start Over
An integrated approach in the discovery and characterization of a novel nuclear protein over-expressed in liver and pancreatic tumors
- Source :
- FEBS Letters; January 2001, Vol. 496 Issue: 2 p109-116, 8p
- Publication Year :
- 2001
-
Abstract
- An integrated approach in protein discovery through the use of multidisciplinary tools was reported. A novel protein, Hcc-1, was identified by analysis of the hepatocellular carcinoma (HCC)-M cell proteome. The assembled EST sequence of the 210 amino acid novel protein was subsequently confirmed by rapid amplification of cDNA ends (RACE). A total of 687 bp at the 5′ untranslated region of Hcc-1 was identified. Promoter activity and several upstream open reading frames (uORFs) were demonstrated at this region. Bioinformatics prediction showed that the first 42 amino acids of the protein is a SAP domain with sequence matches to hnRNP from various vertebrate species. The Hcc-1 protein was localized to the cell nucleus while the gene was localized to chromosome 7q22.1. Hcc-1 cDNA level was increased in pancreatic adenocarcinoma. The level was also increased in well-differentiated hepatocellular carcinoma but decreases as the carcinoma progressed to a poorly differentiated stage.
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 496
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- FEBS Letters
- Publication Type :
- Periodical
- Accession number :
- ejs2508957
- Full Text :
- https://doi.org/10.1016/S0014-5793(01)02409-7