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Inter-domain cross-talk controls the NifA protein activity of Herbaspirillum seropedicae
- Source :
- FEBS Letters; January 2001, Vol. 508 Issue: 1 p1-4, 4p
- Publication Year :
- 2001
-
Abstract
- Herbaspirillum seropedicaeis an endophytic diazotroph, which colonizes sugar cane, wheat, rice and maize. The activity of NifA, a transcriptional activator of nifgenes in H. seropedicae, is controlled by ammonium ions through a mechanism involving its N-terminal domain. Here we show that this domain interacts specifically in vitro with the N-truncated NifA protein, as revealed by protection against proteolysis, and this interaction caused an inhibitory effect on both the ATPase and DNA-binding activities of the N-truncated NifA protein. We suggest that the N-terminal domain inhibits NifA-dependent transcriptional activation by an inter-domain cross-talk between the catalytic domain of the NifA protein and its regulatory N-terminal domain in response to fixed nitrogen.
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 508
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- FEBS Letters
- Publication Type :
- Periodical
- Accession number :
- ejs2509518
- Full Text :
- https://doi.org/10.1016/S0014-5793(01)03017-4