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Engineering characteristics of a single‐use stirred bioreactor at bench‐scale: The Mobius CellReady 3L bioreactor as a case study

Engineering characteristics of a single‐use stirred bioreactor at bench‐scale: The Mobius CellReady 3L bioreactor as a case study

Authors :
Kaiser, Stephan C.
Eibl, Regine
Eibl, Dieter
Source :
Engineering in Life Sciences; August 2011, Vol. 11 Issue: 4 p359-368, 10p
Publication Year :
2011

Abstract

Stirred single‐use bioreactors can be used as substitutes for their conventional counterparts made of glass or stainless steel in the development and production of biopharmaceuticals wherever possible. Various studies have confirmed their comparability in cell growth as well as in product quantity and quality. However, information about their engineering characteristics is still rare. This study focuses on the stirred Mobius®CellReady 3L bioreactor. The main engineering parameters for typical operation conditions used in animal cell cultivations are presented for the first time. Numerical simulations with a commercial CFD package (Fluent 6.3) were accomplished to obtain data on the single‐ and multi‐phase fluid flow, power input, mixing time and oxygen mass transfer. The results, which were compared with data from experiments and from the literature, reveal the suitability of the Mobius®CellReady 3L bioreactor for cell expansion and protein production with animal cell cultures. Furthermore, the data enable comparisons with other single‐use and reusable cell culture bioreactors at bench‐scale.

Details

Language :
English
ISSN :
16180240 and 16182863
Volume :
11
Issue :
4
Database :
Supplemental Index
Journal :
Engineering in Life Sciences
Publication Type :
Periodical
Accession number :
ejs25635480
Full Text :
https://doi.org/10.1002/elsc.201000171