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Molecular Cloning and Partial DNA Sequencing of the Collagenase Gene of Vibrio alginolyticus
- Source :
- Microbiology and Immunology; December 1990, Vol. 34 Issue: 12 p977-984, 8p
- Publication Year :
- 1990
-
Abstract
- DNA fragments Vibrio alginolyticuschemovar iophagus, at least 7 kb in length, were ligated to Escherichia coliexpression vectors. Three clones of Escherichia coliHB101 (pLCO‐1, pLCO‐2, pLCO‐3) were obtained by the colony immunoblotting method using anti‐collagenase antibody. In Escherichia coli, all these genes produced collagenase antigens which were detected with Western blotting. The amino acid sequence of chemically purified collagenase fragments was also analyzed. An approximately 2.5 kb DNA fragment of the pLCO‐1 clone was sequenced, and we found that portions of the deduced amino acid sequence were completely identical with the corresponding amino acid sequence of the chemically analyzed fragments. Therefore, it is highly probable that the gene studied in the present experiment is truly a collagenase structural gene.
Details
- Language :
- English
- ISSN :
- 03855600 and 13480421
- Volume :
- 34
- Issue :
- 12
- Database :
- Supplemental Index
- Journal :
- Microbiology and Immunology
- Publication Type :
- Periodical
- Accession number :
- ejs31451886
- Full Text :
- https://doi.org/10.1111/j.1348-0421.1990.tb01519.x