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Improved Physical and Biochemical Features of a Collagen Membrane by Conjugating with Soluble Egg Shell Membrane Protein
- Source :
- Bioscience, Biotechnology, and Biochemistry; January 2006, Vol. 70 Issue: 4 p865-873, 9p
- Publication Year :
- 2006
-
Abstract
- Soluble egg shell membrane protein (SEP) prepared from the egg shell membrane by performic acid oxidation and pepsin digestion was applied to prepare a membrane conjugated with pepsin-solubilized collagen (PSC) by cross-linking with water-soluble carbodiimide. SEP enabled a conjugated membrane to be constructed with a well-developed network structure having thicker collagen fibrils and higher stability than the PSC membrane alone, as indicated by reduced solubility and collagenase-digestion, increased denaturation temperature, and superior flexibility. The SEP-PSC membrane (SEP:PSC of 1:1, w/w) had lower sweatiness than the PSC membrane. Fibroblasts and keratinocytes adhered to the SEP-PSC-coated wells (SEP:PSC of 1:10, w/w) as a fundamental model of an artificial skin similar to that to the PSC-coated wells due to similar expression level of several cell-adhesion proteins. An SEP-PSC coating accelerated the cell growth of fibroblasts, whereas the cell growth of keratinocytes did not change, or decreased slightly due to more rapid differentiation indicated by a more substantial expression of differentiation marker proteins than a PSC-coating without SEP.
Details
- Language :
- English
- ISSN :
- 09168451 and 13476947
- Volume :
- 70
- Issue :
- 4
- Database :
- Supplemental Index
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Publication Type :
- Periodical
- Accession number :
- ejs32911918
- Full Text :
- https://doi.org/10.1271/bbb.70.865