Cloning and Expression Analysis of MloGene from Pericallishybrida B. Nord.

The full-length Mlogene was obtained by reverse transcription polymerase chain reaction (RT-PCR) and RACE. The result of sequence analysis indicated that Mlogene from Pericallishybrida B. Nord. contained about 1 296 bp open reading frame and encoded 431 amino acids. According to the comparison of the exogenous gene sequences by BLAST analysis and phylogenetic analysis, Mlogene shared over 85% nucleotide homology and 98% amino acid homology. Finally, through semi-quantitative-PCR and fluorescence quantitative analysis, we found that Mlogene showed the highest expression levels in leaves and the lowest in roots after inoculated with powdery mildew pathogen for different days.